Autoimmun Diagnostika GmbH AID-Autoimmun Diagnostika GmbH
 
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Method

 

All AID diagnostic kits are based on the Reverse Hybridisation technique, a method designed for rapid and reproducible analysis of polymorphisms, mutations and infections.

 

Advantages of the AID Reverse Hybridisation Assays: 		 

 

  • Fast, accurate, reproducible
  • Easy to perform
  • Low cost method with standard lab equipment
  • Low-, medium- and high-throughput applications (Ask for Blot-Automation)
  • Different genes/pathogens on one filter
  • Analysis with the AID GenoBlot Scanning System

  
 
graphic: the technic of Reverse hybridization

A AID hybridisation filter contains a variable number of reaction zones (fixed DNA probes).
Test principle

The test is carried out in two steps:

1.  Amplification of biotinylated alleles from genomic DNA by standard PCR*

2.  Hybridisation of the PCR-products to strips precoated with oligonucleotide probes specific for the alleles.

A highly specific washing procedure ensures that only hybrids with 100 % complementary sequences survive. Detection is subsequently achieved by the sequential addition of streptavidin-coupled alkaline phosphatase and the substrate BCIP/NBT to give a coloured product.
The interpretation of the strips is done by template or automated with the GenoBlot Scanning System.


 
* The polymerase chain reaction (PCR) is patented by the company Hoffmann-La-Roche.

reverse hybridisation 		 
Conjugation control:
Indicates the performance of the hybridisation reaction and must be developed.
 
Specificity control:
Checks the stringency of the reaction and should never be developed.
 
Sensitivity control:
Documents the optimal sensitivity of the reaction and should always be developed.
 
Reaction zones (variable):
These DNA zones are variable in number on each AID Kit. Depending on the genotyp of the patient or the type of infection some zones are developed, while some are not.
 
  Hematology
Faktor V / Faktor II + HR 2
Faktor V/II + MTHFR
MTHFR
Hereditery Hemochromatosis
  Immunology
HLA-B*27 + CYP2D6*4
HLA-DRB1 Shared epitope
Celiac disease(HLA-DQ and DR4)
Celiac disease(HLA-DQ2, DQ8 and DR4)
IDDM
  Infection
Herpes simplex
Periodontitis++
HPV-typing
HPV-screening
STD
CAP Bacterial
CAP Viral
CAP Resistance
MRSA combi
CAP Juvenile
Influenza-typing
Influenza-screening
Influenza-H5N1
  Metabolism
Apolipoprotein E+B (ApoE+ApoB)
Osteoporosis
Alpha-1-Antitrypsin
Lactose intolerance
Fructoseintolerance NEW!
  Pharmacogenetics
Cytochrom P450 CYP2C19
Cytochrom P450 CYP2C9
Glutathion-S-Transferase
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