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HLA-DRB1 Shared epitope
QKRAA/QRRAA/RRRAA

RDB2035

Reverse hybridization kit for the detection of the shared epitope QKRAA/QRRAA/RRRAA of rheumatoid arthritis in all known HLA-DRB1 alleles; differentiation between homozygot and heterozygot


>> Nomenclature and clinical relevance, References
>> Principles of the test
Examples for the detection



  

Examples for the detection of the shared epitope in different alleles of the HLA-DRB1 gene

The shared epitope nitrocellulosestrip is coated with 4 controls and 10 DRB1 specific geneprobes. The kit contains comprehensive charts and a template for the evaluation and interpretation of the results.

The shared epitope strip:

  
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Please click on the image to see enlarged version.



Results:

  
1. First hybridization: Allel 1: no DRB1*01 or DRB1*04 allele
Allel 2: no DRB1*01 or DRB1*04 allele
2. First hybridization:
    Second hybridization:		 Allele 1: DRB1*04-QKRAA
Allele 2: no further shared epitope allele
3. First hybridization:
    Second hybridization:		 Allele 1: DRB1*01-QRRAA
Allele 2: no furhter shared epitope allele
4. First hybridization: Allele 1: DRB1*01-QRRAA
Alle e2: DRB1*04-QKRAA
5. First hybridization: Allele 1: DRB1*04-QKRAA
Allele 2: DRB1*04-QRRAA
6. First hybridization:
    Second hybridization:		 Allele 1: DRB1*04-QKRAA
Allele 2: DRB1*04-QKRAA
7. First hybridization:
    Second hybridization:		 Allele 1: DRB1*04-QRRAA
Allele 2: no further shared epitope allele
8. First hybridization: Allele 1: DRB1*04-QRRAE (it is not a shared epitope allele)
Allele 2: no DRB1*01 or DRB1*04 allele
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>> Nomenclature and clinical relevance, References
>> Principles of the test		  
Infection
Human Genetics
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Faktor V/II + MTHFR
MTHFR
Hereditary Hemochromatosis
  Immunology
HLA-B*27 + CYP2D6*4
HLA-DRB1 Shared epitope
Celiac disease(HLA-DQ2, DQ8 and DR4)
  Metabolism
Apolipoprotein E+B (ApoE+ApoB)
Osteoporosis
Lactose intolerance
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  Pharmacogenetics
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Cytochrom P450 CYP2C9 + VKORC1
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NAT-2
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