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HLA-DRB1 Shared epitope
QKRAA/QRRAA/RRRAA


Reverse hybridization kit for the detection of the shared epitope QKRAA/QRRAA/RRRAA of rheumatoid arthritis in all known HLA-DRB1 alleles; differentiation between homozygot and heterozygot

RDB2035
>> Nomenclature and clinical relevance, References
>> Principles of the test
Examples for the detection



  
Examples for the detection of the shared epitope in different alleles of the HLA-DRB1 gene

 The shared epitope nitrocellulosestrip is coated with 4 controls and 10 DRB1 specific geneprobes. The kit contains comprehensive charts and a template for the evaluation and interpretation of the results.

The shared epitope strip:

  
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Please click on the image to see enlarged version.



Results:

 
1. First hybridization: Allel 1: no DRB1*01 or DRB1*04 allele
Allel 2: no DRB1*01 or DRB1*04 allele
2. First hybridization:
    Second hybridization:		 Allele 1: DRB1*04-QKRAA
Allele 2: no further shared epitope allele
3. First hybridization:
    Second hybridization:		 Allele 1: DRB1*01-QRRAA
Allele 2: no furhter shared epitope allele
4. First hybridization: Allele 1: DRB1*01-QRRAA
Alle e2: DRB1*04-QKRAA
5. First hybridization: Allele 1: DRB1*04-QKRAA
Allele 2: DRB1*04-QRRAA
6. First hybridization:
    Second hybridization:		 Allele 1: DRB1*04-QKRAA
Allele 2: DRB1*04-QKRAA
7. First hybridization:
    Second hybridization:		 Allele 1: DRB1*04-QRRAA
Allele 2: no further shared epitope allele
8. First hybridization: Allele 1: DRB1*04-QRRAE (it is not a shared epitope allele)
Allele 2: no DRB1*01 or DRB1*04 allele
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You can find a comprehensive list of all known HLA allele in
http://www.anthonynolan.com/HIG/index.html


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>> Principles of the test		  
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