Glutathion-S-TransferaseReverse hybridization kit for the determination of the polymorphism of Glutathion-S-Transferase (GST) M1 and T1 RDB2090 |
||
|
Clinic Glutathione-S-transferases (GST) are a multigen family of enzymes involved in detoxification and, sometimes, activation of a wide variation of exogene and endogene substances. In human they make up to 4% of total soluble protein in liver and catalyze the conjugation of glutathion to numerous potentially genotoxic xenobiotics, including aliphatic heterocyclic radicals, epoxides and arene oxides. The enzymes detoxify carcinogenic polycyclic aromatic hydrocarbons and conjugate isothiocyanates by transducing them in less reactive and more water soluble products (1). Six classes of enzymes have been described as a, �, p, t, z, w (GST-A, -M, -P, -T, -Z, -O). Various polymorphisms of the GST genes can be found, which results in reduced activity of the GST enzymes. Subjects with different GST genotype may therefore have different susceptibilities to environmental exposures and may be unable to eliminate electrophilic carcinogenes as efficiently. This may increase the risk of somatic mutations leading to tumor formation (2). GST-M1 and GST-T1 deletion genotypes For GST-M1 and GST-T1 gene deletion genotypes are described and individuals with homozygous deletions of GST-M or GST-T (called null genotype) lack any functional GST-M1 or GST-T1 protein. The frequency of these null genotypes in the caucasian population is 50% for GST-M1 and about 20% for GST-T1. In various studies different associations between cancer and GST genotypes has been observed. Allthough interpretation of such studies is very difficult because of interactions in detoxification of large numbers of GST isozymes as well as other biotransforming enzymes like cytochromes P450 and N-Acetyltransferases. Beside of direct association with diseases the combination of both null-genotypes GST-M1 and GST-T1 was found to be associated with unresponsiveness to primary chemotherapy (3). |
||
|
||
|
References
|
 |
AID
GmbH Ebinger Strasse 4 D-72479 Straßberg |
