| Anti-Helicobacter
pylori-IgG, -IgA, and -IgM-Immunoblots Here you see an Anti-Helicobacter pylori-IgG-Immunoblot scanned with the AID-BlotScanning System
Helicobacter pylori Cut off: Standard settings:
Freely adjustable as required. Antigen: Purified complete Helicobacter pylori antigen. The following bands are characterised:
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| Specificity: |
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| 120
kD (cagA gen product) This protein is highly specific to H. pylori, although it is not expressed by all H. pylori strains. Its function has not yet been discovered. The cagA genetic product is an important pathogenic factor. |
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| 87 kD
(vacA gen product) This protein is highly specific to H. pylori. It is the so-called vacuolizing cytotoxin, the second most important pathogenic factor of H. pylori. |
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| 67 kD This protein is a flagellin protein, which shows a strong cross-reaction to other bacteria also containing a flagellin. An immunoresponse to this protein is therefore very frequent and cannot be considered specific. |
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| 66 kD
and 29 kD These proteins are sub-units of the urease enzyme. They are highly specific to H. pylori. |
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| 25, 19,
17 und 14 kD Little is known about these proteins, but literature reports the 19 kD protein to be highly specific to H. pylori. |
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| Comments:
The isolated appearance of antibodies against gegen cagA und vacA seems to be the remaining titres of a previous infection. These antibodies persist for a long time. |
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| Interpretation: IgG results positive: If 2 of the highly specific bands (120, 87, 66, 29, 19 kD) are marked. Or at least 3 of the characterised bands (without 67 kD) are marked. IgG results uncertain: If one of the pathogenic factors (120 kD, 87 kD) or one of the highly specific urease bands (66 kD, 29 kD) is marked. Or at least 2 of the characterised bands are present (without 67 kD). IgA results positive: If 2 of the following bands are marked: 120, 87, 66, 29, 25, 19, 17, 14 kD. IgA results uncertain: If one of the highly specific bands is marked (120, 87, 66, 29, 19 kD). Further assistance in interpretation: The most important additional information in the immunoblot is the examination of the pathogenic factors (cagA and vacA). CagA and vacA- positive H. pylori strains are highly virulent. Patients with antibodies against vacA and/ or cagA run a higher risk of contracting a gastric ulcer than patients infected with a vacA or cagA negative strain. |
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AID
GmbH Ebinger Strasse 4 D-72479 Straßberg |
