| Anti-Yersinia
enterocolitica-IgG- and -IgA-Immunoblots Here you see an Anti-Yersinia enterocolitica-IgG-Immunoblot scanned with the AID-BlotScanning System
Yersinia enterocolitica Cut off: Standard settings:
Antigen: Processed calcium deficient Y. enterocolitica cultures (serovar 0:9). |
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The following bands are
characterized:
Specificity: The human pathogenic Yersinia owes its high antigenicity to the so-called ”release proteins” or ”Yersinia outer proteins” (Yop). These are extremely specific to human pathogenic Yersinia. |
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| Yop
1 (58 kD) Little is yet known about this protein. It is probably involved in the Yersinia´s active iron intake from its surroundings. |
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| Yop
2a (48 kD) This protein is responsible for the inhibition of thrombocytic aggregation. |
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| Yop
2b (46 kD) This protein makes the Yersinia resistant to phagocytosis. |
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| Yop
3 (40 kD) Is also known as V-antigen. Little is known about the role of this protein. |
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| Yop
4a (36 kD) The function of this protein is also disputed. It is important in interpretation in that, according to literature, the main IgA response is directed against it. |
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| Yop
4b (34 kD) The function of this protein is also disputed. |
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| Yop
5 (25 kD) This protein plays an important role in the cytotoxicity of the Yersinia. |
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| Sp-Protein Nothing is known about this protein. It seems to be specific to Yersinia, is however often marked. Is not included in the interpretation. |
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| Comments:
This test kit has not been approved according to AMG for the diagnosis of yersinia- induced enteritis infections. |
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| Interpretation: IgG results positive: 4 or more Yops marked. IgG results uncertain: 3 Yop’s marked. IgA results positive: 2 or more Yops marked. Further assistance in interpretation: Persistant Yersinia-specific IgA antibodies are usually detected in patients suffering from Yersinia-induced arthritis. Additional antibody reactions in the immunoblot, such as heat shock proteins (at the moment not characterised), point to a chronic inflammation (especially an IgA response). |
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AID
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