Increasing prevalence of resistance against cephalosporins in extended-spectrum ß-lactamase (ESBL)-producing Enterobacteriaceae has continuously been reported over the past years.
Depending on the ESBL type, these b-lactamases have the ability to hydrolyse various penicillins, first-, second- and third-generation cephalosporins, and aztreonam (but not cephamycins or carbapenems). Currently, the most prevalent ESBLs include TEM, SHV and CTX-M enzyme types.
Phenotypic susceptibility testing for ESBLs may be complicated by the presence of multiple ß-lactamases in a single bacterial strain, e.g. ESBLs, AmpCs and carbapenemases, and by different levels of b-lactamase gene expression.Genotypic tests have the potential to accurately identify different ß-lactamase genes
and mutations that result in ESBLs
- Developed for rapid detection of ESBL-genes within 5 hours
- Detection of the most important mutations in TEM and SHV as soon as CTX-M and the Klebsiella pneumoniae Carbapenemase (KPC)
- For culture and clinical specimens
- Results within 4 hours
- Suited for automated systems
- Control bands on every strip shows correct DNA isolation, amplification and hybridization
- Evaluation and documentation with AID scanner and software