Accurate detection of celiac disease risk alleles
Detection of DQ2 and DQ8 HLA heterodimers may serve as a diagnostic marker for celiac disease as HLA-B*27 does for ankylosing spondylitis.
In individuals with gastrointestinal malfunction, the presence of DQ2 and/or DQ8 would increase the chance that the person has celiac disease whereas the absence of this molecule would greatly argue against this assumption.
In a recent European report, only 0.5% of celiac patients lacked both DQ2 and DQ8. As little as 0.3% of tissue transglutaminase autoantibody-positive individuals do not have DQ2 or DQ8. The majority of DQ2- and DQ8-negative celiac disease patients did in fact carry one half of the DQ2 heterodimer, often in the form DR7 (DQB1*0201)
The AID Celiac Disease Kit enables the detection of genes for predisposition heterodimers HLA-DQ(α1*0501, β1*0201) and HLA-DQ(α1*03, β1*0302) as well as DR4 allele.
- Accurate detection of celiac disease risk alleles
- Diagnostic is independent from a IgA antibody deficiency
- Detects patients with latent or potential celiac disease
- Differentiation between homozygous and heterozygous genotype
- PCR with subsequent lineprobe assay
- Specimen: human DNA isolated from buccal swab, Citrate- or EDTA-blood or biopsy
- Control bands on every strip show correct DNA isolation, amplification and hybridization
- Results within 4 hours
- Suited for automated systems
- Evaluation and documentation with AID Scanning system
- Art.no.: RDB 2105