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Human Genetics

IMMUNOLOGY

RDB2035 Shared Epitope

Rheumatoid arthritis (RA) or chronic polyarthritis is an intermittent systemic autoimmune disease which occurs in approx. 1% of the population. The etiology of the disease is unknown.

There are great differences in progression of the disease in different patients. Therefor a early diagnosis is essential for optimal therapy to avoid long-term damages.

It has been shown by studies that there is a genetic disposition for RA caused by several alleles of the HLA-DR region. RA is associated with the HLA- DRB1 alleles coding for amino acid motives QKRAA (*0401), QRRAA (*0404, *0405, *0408, *0101, *0102, *1402) or RRRAA (*1001), named „rheumatoid epitope" or „Shared Epitope“. About 80-90% of all Caucasian RA patients are heterozygous or homozygous for one of these HLA-DR-Shared Epitope alleles.

AID Shared Epitope Kit enables the detection of HLA-risk alleles for RA.

 

 

Characteristics

  • Detection of Shared Epitope Genotype by screening of HLA-DRB1 allele and subtyping of DR1 and DR4
  • Differentiation between homozygous and heterozygous genotype
  • PCR with subsequent lineprobe assay
  • Specimen: human DNA isolated from buccal swab, Citrate- or EDTA-blood or biopsy
  • Control bands on every strip show correct DNA isolation, amplification and hybridization
  • Results within 4 hours
  • Suited for automated systems
  • Evaluation and documentation with AID Scanning system

 

Reader Systems

AID Scanner