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Human Genetics

PHARMACOGENETICS

RDB2265 BRAF

BRAF is a Serotonin/Threonine protein kinase downstream of KRAS in der MAPK-ERK-signal pathway between EGF receptor and cell nucleus. Somatic mutation of BRAF gene can be found in up to 60% of melanoma biopsy and also in other tumor tissue.

Beside a main mutation V600E with a frequency of 70-90% there are some other, less frequent mutations all result in a permanent ON signal of BRAF and therefor a permanent stimulus for cell proliferation.

AID BRAF Kit enables sensitive screening for most frequent mutations in exon 15 of BRAF gene.

 

 

 

Characteristics

  • Sensitive detection of the most frequent mutations of BRAF gene
    Sensitivity: down to 4% mutation vs WT
  • PCR with subsequent lineprobe assay
  • Specimen: DNA from fresh or FFPE biopsy
  • Control bands on every strip show correct DNA isolation, amplification and hybridization
  • Results within 4 hours
  • Suited for automated systems
  • Evaluation and documentation with AID Scanning system

 

Reader Systems

AID Scanner